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　　“精要速覽系列”（InstantNotesSeries)叢書(shū)是國(guó)外教材“Bestseller”榜的上榜教材。該系列叢書(shū)結(jié)構(gòu)新穎，視角獨(dú)特；重點(diǎn)突出，脈絡(luò)分明；圖表清晰，英文自然易懂，被國(guó)內(nèi)許多所重點(diǎn)院校選作雙語(yǔ)教材。　　分子生物學(xué)第四版是在第三版的基破上修訂而成的�！斗肿由飳W(xué)（導(dǎo)讀版，第四版）》共分19章，分別是：信息大分子，核酸的性質(zhì)，原核與真核生物的染色體結(jié)構(gòu)，DNA復(fù)制，DNA損傷、修復(fù)與重組，原核生物的轉(zhuǎn)錄，原核生物的轉(zhuǎn)錄調(diào)控，真核生物的轉(zhuǎn)錄，真核生物的轉(zhuǎn)錄調(diào)控，RNA加工與核糖核蛋白復(fù)合體，遺傳密碼與tRNA，蛋白質(zhì)合成，噬菌體與真核生物病毒，細(xì)胞周期與癌癥，基因操作技術(shù)，克隆載體，基因文庫(kù)與篩選，克隆DNA的分析與應(yīng)用，功能基因組學(xué)及其新技術(shù)。　　Alexander McLennan, Andy Bates，Phil Turner, Mike White, Instant Notes in Molecular Biology, 4th edition. ISBN 9780415684163. Copyright？2012 by Taylor & Francis Group LLC. All Rights Reserved. Authorized translation from the English language edition published by CRC Press，a member of the Taylor & Francis Group LLC. 　　Licensed for sale in the Mainland of China only, booksellers found selling this title outside the Mainland of China will be liable to prosecution.

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目錄第四版前言縮略詞 A 信息大分子 1 A1 信息加工與分子生物學(xué) 1 A2 核酸結(jié)構(gòu)與功能 1 A3 蛋白質(zhì)結(jié)構(gòu)與功能 2 A4 大分子組裝 4 A5 蛋白質(zhì)分析方法 4 B 核酸的性質(zhì) 38 B1 核酸的理化特性 38 B2 核酸的光譜學(xué)和熱力學(xué)特性 38 B3 DNA超螺旋 39 C 原核與真核生物的染色體結(jié)構(gòu) 52 C1 原核生物的染色體結(jié)構(gòu) 52 C2 染色質(zhì)結(jié)構(gòu) 52 C3 真核生物的染色體結(jié)構(gòu) 53 C4 基因組復(fù)雜度 54 D DNA復(fù)制 75 D1 DNA復(fù)制概述 75 D2 細(xì)菌的DNA復(fù)制 75 D3 真核生物的DNA復(fù)制 76 E DNA損傷、修復(fù)與重組 91 E1 DNA損傷 91 E2 誘變 91 E3 DNA修復(fù) 92 E4 重組與易位 93 F 原核生物的轉(zhuǎn)錄 112 F1 轉(zhuǎn)錄的基本原理 112 F2 大腸桿菌RNA聚合酶 112 F3 大腸桿菌σ70啟動(dòng)子 113 F4 轉(zhuǎn)錄的起始、延伸與終止 113 G 原核生物的轉(zhuǎn)錄調(diào)控 129 G1 乳糖操縱子 129 G2 色氨酸操縱子 129 G3 不同σ因子和非編碼RNA對(duì)轉(zhuǎn)錄的調(diào)控 130 H 真核生物的轉(zhuǎn)錄 145 H1 三種RNA聚合酶：特性及其功能 145 H2 RNA聚合酶Ⅰ基因：核糖體重復(fù) 145 H3 RNA聚合酶Ⅲ基因：5S基因與tRNA基因的轉(zhuǎn)錄 146 H4 RNA聚合酶Ⅱ基因：?jiǎn)?dòng)子與增強(qiáng)子 147 H5 通用轉(zhuǎn)錄因子與RNA聚合酶Ⅱ的起始 148 I 真核生物的轉(zhuǎn)錄調(diào)控 167 I1 真核生物的轉(zhuǎn)錄因子 167 I2 轉(zhuǎn)錄調(diào)控案例 168 J RNA加工與核糖核蛋白復(fù)合體 183 J1 rRNA加工與核糖體 183 J2 tRNA及其他小RNA的加工 184 J3 mRNA加工、hnRNP和snRNP 185 J4 可變mRNA加工 186 K 遺傳密碼與tRNA 209 K1 遺傳密碼 209 K2 tRNA結(jié)構(gòu)與功能 210 L 蛋白質(zhì)合成 223 L1 蛋白質(zhì)合成概述 223 L2 蛋白質(zhì)合成機(jī)制 223 L3 真核生物蛋白質(zhì)合成的起始 224 L4 翻譯調(diào)控與翻譯后加工 225 M 噬菌體與真核生物病毒 249 M1 病毒簡(jiǎn)介 249 M2 噬菌體 249 M3 DNA病毒 250 M4 RNA病毒 250 N 細(xì)胞周期與癌癥 268 N1 細(xì)胞周期 268 N2 癌基因 269 N3 腫瘤抑制基因 269 N4 凋亡 270 O 基因操作技術(shù) 289 O1 DNA克隆概述 289 O2 質(zhì)粒DNA的制備 289 O3 限制性酶與電泳 290 O4 連接、轉(zhuǎn)化與重組子分析 290 P 克隆載體 313 P1 質(zhì)粒載體的設(shè)計(jì) 313 P2 噬菌體載體、黏粒、YAC及BAC 313 P3 真核生物載體 314 Q 基因文庫(kù)與篩選 334 Q1 基因組文庫(kù) 334 Q2 cDNA文庫(kù) 334 Q3 篩選流程 335 R 克隆DNA的分析與應(yīng)用 346 R1 克隆的鑒定 346 R2 核酸測(cè)序 346 R3 聚合酶鏈反應(yīng) 347 R4 克隆基因的分析 348 R5 克隆基因的誘變 349 S 功能基因組學(xué)及其新技術(shù) 374 S1 組學(xué)概述 374 S2 基因表達(dá)的整體分析 374 S3 蛋白質(zhì)組學(xué) 375 S4 細(xì)胞與分子成像 376 S5 基因轉(zhuǎn)移與干細(xì)胞技術(shù) 377 S6 生物信息學(xué) 377 S7 系統(tǒng)與合成生物學(xué) 378 進(jìn)一步閱讀文獻(xiàn) 419 索引 428 Contents Preface to the fourth edition Section A – Informational macromolecules A1 Information processing and molecular biology 6 A2 Nucleic acid structure and function 9 A3 Protein structure and function 17 A4 Macromolecular assemblies 27 A5 Analysis of proteins 31 Section B – Properties of nucleic acids B1 Chemical and physical properties of nucleic acids 40 B2 Spectroscopic and thermal properties of nucleic acids 44 B3 DNA supercoiling 47 Section C – Prokaryotic and eukaryotic chromosome structure C1 Prokaryotic chromosome structure 56 C2 Chromatin structure 59 C3 Eukaryotic chromosome structure 64 C4 Genome complexity 70 Section D – DNA replication D1 DNA replication: an overview 77 D2 Bacterial DNA replication 82 D3 Eukaryotic DNA replication 87 Section E – DNA damage, repair, and recombination E1 DNA damage 94 E2 Mutagenesis 98 E3 DNA repair 102 E4 Recombination and transposition 107 Section F – Transcription in bacteria F1 Basic principles of transcription 115 F2 Escherichia coli RNA polymerase 118 F3 The E. coli δ70 promoter 121 F4 Transcription initiation, elongation, and termination 124 Section G – Regulation of transcription in bacteria G1 The lac operon 132 G2 The trp operon 136 G3 Transcriptional regulation by alternative δ factors and RNA 141 Section H – Transcription in eukaryotes H1 The three RNA polymerases: characterization and function 149 H2 RNA Pol I genes: the ribosomal repeat 152 H3 RNA Pol III genes: 5S and tRNA transcription 156 H4 RNA Pol II genes: promoters and enhancers 160 H5 General transcription factors and RNA Pol II initiation 163 Section I – Regulation of transcription in eukaryotes I1 Eukaryotic transcription factors 170 I2 Examples of transcriptional regulation 177 Section J – RNA processing and RNPs J1 rRNA processing and ribosomes 187 J2 tRNA and other small RNA processing 193 J3 mRNA processing, hnRNPs, and snRNPs 197 J4 Alternative mRNA processing 204 Section K – The genetic code and tRNA K1 The genetic code 211 K2 tRNA structure and function 216 Section L – Protein synthesis L1 Aspects of protein synthesis 227 L2 Mechanism of protein synthesis 231 L3 Initiation in eukaryotes 238 L4 Translational control and post-translational events 243 Section M – Bacteriophages and eukaryotic viruses M1 Introduction to viruses 252 M2 Bacteriophages 255 M3 DNA viruses 260 M4 RNA viruses 264 Section N – Cell cycle and cancer N1 The cell cycle 271 N2 Oncogenes 276 N3 Tumor suppressor genes 281 N4 Apoptosis 285 Section O – Gene manipulation O1 DNA cloning: an overview 292 O2 Preparation of plasmid DNA 297 O3 Restriction enzymes and electrophoresis 301 O4 Ligation, transformation, and analysis of recombinants 306 Section P – Cloning vectors P1 Design of plasmid vectors 316 P2 Bacteriophages, cosmids, YACs, and BACs 321 P3 Eukaryotic vectors 329 Section Q – Gene libraries and screening Q1 Genomic libraries 336 Q2 cDNA libraries 339 Q3 Screening procedures 343 Section R – Analysis and uses of cloned DNA R1 Characterization of clones 350 R2 Nucleic acid sequencing 354 R3 Polymerase chain reaction 360 R4 Analysis of cloned genes 366 R5 Mutagenesis of cloned genes 370 Section S – Functional genomics and the new technologies S1 Introduction to the ’omics 380 S2 Global gene expression analysis 384 S3 Proteomics 391 S4 Cell and molecular imaging 396 S5 Transgenics and stem cell technology 400 S6 Bioinformatics 404 S7 Systems and synthetic biology 413 Further reading 419 Index 428

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